Open AccessGenotypic characterization of Echinococcus granulosus in Iranian goats
Author(s) -
Mohammad Reza Youssefi,
Rabeeh Tabaripour,
Vahid Fallah Omrani,
Adel Spotin,
Behzad Esfandiari
Publication year - 2013
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(13)60085-7
Subject(s) - echinococcus granulosus , genotype , biology , veterinary medicine , mitochondrial dna , echinococcosis , strain (injury) , gene , zoology , genetics , anatomy , medicine
Objective: To isolate and characterize the genotype of Echinococcus granulosus (E. granulosus)\udfrom goats in Mazandaran Province, Northern Iran.\udMethods: A total of 120 goats were screened from abattoirs of Mazandaran Province, Northern\udIran. Forty out of 120 samples were infected with cystic echinococcosis and 29 out of 40 infected\udsamples were fertile hydatid cysts (containing protoscolices) which were collected from the livers\udand lungs of infected goats. DNA samples were extracted from the protoscolices and characterized\udby mitochondrial DNA sequencing of part of the mitochondrial cytochrome C oxidase subunit 1\udgene.\udResults: Sequences analysis of nine fertile hydatid cysts indicated that all isolated samples were\udinfected with the G1 sheep strain and two sequences were belonged to G1 4 and G1c microvarients\udof the G1 genotype.\udConclusions: The results showed that goats act as alternative intermediate hosts for sheep strain.\udG1 genotype seems to be the main route of transmission and it should be considered in further\udstudies