Open AccessAntioxidant and anti-inflammatory activity of Polygonatum sibiricum rhizome extracts
Author(s) -
Trishna Debnath,
Sa Ra Park,
Da Hey Kim,
Jeong Eun Jo,
Beong Ou Lim
Publication year - 2013
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(13)60074-2
Subject(s) - chemistry , rhizome , antioxidant , nitric oxide , reactive oxygen species , dpph , nitric oxide synthase , anti inflammatory , radical , biochemistry , distilled water , traditional medicine , tumor necrosis factor alpha , pharmacology , biology , immunology , chromatography , organic chemistry , medicine
Objective: To investigate the antioxidant and anti-inflammatory activities of distilled water\udextract of fresh Polygonatum sibiricum (P. sibiricum) rhizome.\udMethods: The extracts were tested for antioxidant activity by using DPPH (1,1-diphenyl-2-picrylhydrazyl), and hydroxyl (OH•) radicals scavenging activity. The level of intracellular reactive\udoxygen species (ROS) was determined in H2O2 treated chang liver cells. Anti-inflammatory activity\udwas evaluated by the inhibition of nitric oxide (NO), inducible nitric oxide synthase (iNOS), as well\udas tumor necrosis factor-alpha (TNF-α) protein expression in a lipopolysaccharide stimulated\udRaw 264.7 murine macrophages cell line.\udResults: P. sibiricum water extracts scavenged DPPH, OH• radicals and decreased the level ROS.\udThe extracts reduced NO reduction and inhibited the expression of iNOS and TNF-α proteins.\udConclusions: The findings indicate that water extracts of P. sibiricum could be considered as\udnatural antioxidants and anti-inflammatory agents for food and drug industries