Open AccessEvaluation of antioxidant potential of leaves of Leonotis nepetifolia and its inhibitory effect on MCF7 and Hep2 cancer cell lines
Author(s) -
Usharani Veerabadran,
V. Anuradha,
Aroumougame Souprayane,
N. Mathivanan,
Dhanalakshmi Perumal,
E Sagadevan,
Sindhu Sareen,
Vadivelu Devaraj,
P Arumugam
Publication year - 2013
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(13)60053-5
Subject(s) - dpph , phytochemical , chemistry , antioxidant , cytotoxicity , mtt assay , dna fragmentation , viability assay , mcf 7 , traditional medicine , phenols , fragmentation (computing) , biochemistry , chromatography , cell growth , cancer cell , cell , in vitro , apoptosis , biology , cancer , programmed cell death , human breast , medicine , genetics , ecology
Objective: To investigate the antioxidant and antiproliferative potential of Leonotis nepetifolia\ud(L. nepetifolia) leaves. Methods: The leaves of L. nepetifolia were subjected to extraction using\udthree different solvents and the antioxidant potential of those extracts were tested by using\udvarious in vitro assays. Further, the best screened extract was analyzed for its phytochemical\udprofile by both qualitative and quantitative assays. In order to determine its anti-proliferative\udactivity, the best screened extract was treated with breast and laryngeal cancer cell lines such as\udMCF-7 cells and Hep2 cells, respectively. The cytotoxicity of the extract was also studied using\udMTT assay. The inhibitory effect of the extract of leaves of L. nepetifolia on the selected cell-line\udDNA was determined by DNA fragmentation assay. Also, the extract was subjected to TLC and\udbioautography analysis. Results: The DPPH assay showed methanol extract of L. nepetifolia leaves\udto be more significant in scavenging free radicals with inhibition percentage of 60.57%. From the\uddata obtained, the methanol extract proved to be significant in all anti-oxidant assays and this\udeffect was well comparable with the standard used in the study. The predominant phytochemicals\udsuch as phenols and flavonoids were further quantified as 0.107% and 0.089%. The cytotoxicity\udassay showed that the cell viability increased with increasing concentration of methanol extract.\udIn addition, the extract caused dose dependent damage to the cancer cell lines MCF-7 and Hep2.\udConclusions: Our study suggests that the leaves of L. nepetifolia were significant in scavenging\udfree radicals and causing damage to proliferative cells. Further mechanistic studies would help in\udproving the efficiency of the selected plant under in vivo conditions