Open AccessRapid confirmation of drug susceptibility in Mycobacterium tuberculosis using MPT 64 Ag based test
Author(s) -
Ernest Afu Ochang,
O.O. Oduyebo,
Ifeanyi A Onwuezobe,
Sunday M Obeten,
G. I. Ogban,
U. E. Emanghe
Publication year - 2013
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(13)60042-0
Subject(s) - isoniazid , rifampicin , mycobacterium tuberculosis , tuberculosis , microbiology and biotechnology , drug resistance , antibiotics , virology , medicine , biology , pathology
Objective: To evaluate the possible use of MPT64 based rapid test to detect multi-drug resistant\udMycobacterium tuberculosis (M. tuberculosis) in antibiotic broth dilution cultures. Methods:\udTwenty five isolates of M. tuberculosis whose susceptibility pattern had previously been identified\udby HAIN Genotype MTBDRplus (HainLifecience, Herhen, Germany) were processed and cultured\udaccording to the microscopic-observation drug susceptibility technique. These included 20\udsusceptible, two multi-drug resistant M. tuberculosis and three isoniazid mono-resistant isolates.\udAfter 10-day incubation, aspirates from each well were tested with the MPT64 rapid test. Results:\udThe rapid test correctly identified all 25 isolates and detected rifampicin and isoniazid resistance\udin all but one isoniazid mono-resistant isolate. Conclusions: MPT64 rapid test could be useful in\uddetecting M. tuberculosis and drug resistance from Middlebrook 7H9 antimicrobial broth dilution\udin resource poor settings without an inverted microscope