Open AccessEffects of intravenous palm oil–based lipid nanoemulsion on fat metabolism in rabbits
Author(s) -
Mahdi Jufri,
Norazrina Azmi,
Ahmad Fuad Shamsuddin
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60297-7
Subject(s) - triglyceride , saline , chemistry , lipid metabolism , cholesterol , palm oil , metabolism , endocrinology , medicine , biochemistry , food science
Objective: To investigate the effects of a newly-developed nanoemulsion of palm oil in\udcombination with medium chain triglyceride (MCT) oil (NEMS襅 MCT/LCT20%) on fat metabolism\udin male New Zealand white rabbits. Methods: Six rabbits were divided into two groups of three\udrabbits in each group. NEMS襅 MCT/LCT20%, Lipofundin襅 MCT/LCT 20% (a commerciallyavailable lipid emulsion) and normal saline (control) were administered intravenously in these\udrabbits via the marginal ear vein for 6 h. Cross-over method was used in these rabbits with a\udwash-out period of two weeks in-between infusions. Triglyceride (TG), total cholesterol, low\uddensity lipoprotein and high density lipoprotein cholesterols were determined using enzymatic\udstrip test at 0, 2, 4 and 6 h of infusion. Blood levels of free fatty acid (FFA) were measured at 0\udand 6 h. Results: Serum TG levels increased in these rabbits after 2 and 4 h infusion of NEMS襅\udMCT/LCT20% but returned to normal after 6 h. Concentration of FFA increased in a dose-related\udmanner but remained within the normal range (315-535 毺 mol/L). Similar results were obtained\udwith Lipofundin襅 MCT/LCT20%. All parameters of fat metabolism in these rabbits remained\udunchanged from baseline when normal saline was administered. Changes in the parameters of fat\udmetabolism measured between NEMS襅 MCT/LCT20% and Lipofundin襅 MCT/LCT20% did not show\udany significant difference. Significant difference (P<0.05) was observed in these parameters when\udNEMS襅 MCT/LCT20% was compared to normal saline. Conclusions: Intravenous administration\udof NEMS襅 MCT/LCT20% did not cause a permanent increase in the TG level of rabbits while FFA\udremained within the normal range