Open AccessHemostatic activity screening and skin toxicity of sap of Jatropha multifida L. (Euphorbiaceae) used in traditional medicine (Benin)
Author(s) -
Victorien Doug,
K Robert,
Jacques Doug,
Julien Segbo,
Atègbo Jean-Marc,
Edorh Patrick Aléodjrodo,
Sodipo Olufunkè,
Doug Ferdinand,
Del Carlos,
Frédéric Loko,
K. Dramane
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60293-x
Subject(s) - jatropha curcas , partial thromboplastin time , hemostasis , chemistry , thrombin time , bleeding time , prothrombin time , blood proteins , coagulation , euphorbiaceae , traditional medicine , pharmacology , platelet , medicine , surgery , biochemistry , botany , biology , platelet aggregation
Objective: To evaluate the hemostatic potential of Jatropha multifida (J. multifida)‘s sap as\udtraditional medicine in Benin attributed hemostatic properties to this substance. Methods:\udSeveral hemostatic tests such as blood coagulation time, Quick time, activated cephalin Time,\udtest of milk precipitation and dosage of total proteins of blood samples were performed at 37\ud曟. Skin toxicity tests were also realized on 14 Wistar rats. Results: Prothrombin time revealed\udthat prior to thromboplastin‘s addition, there was precipitation of the plasma in all tubes except\udfor the control tube (T0). After addition of thromboplastin, plasma in control tube coagulated\udin 78 seconds while plasma in tests tubes were not coagulated (>15 min) (P<0.05). The same\udobservations were made for the activated cephalin time for which prior to addition of cephalin\udactivator, there was precipitation of plasma in all tubes except for the control tube (T0). After\udaddition of cephalin activator, plasma in control tube was coagulated within 43.33 seconds while\udplasma in tests tubes were not coagulated (>15 min) (P<0.05). Blood coagulation time decreased\udregardless of the administered dose of sap. Time of milk‘s precipitation showed the sap rushes\udmilk proteins. This was confirmed by the determination of total proteins in serum, proteins which\uddecreased by over 40% with 1/4 dilution for example (P<0.05). Sap hasn’t also any irritant effect on\udthe skin of animals (the irritation score obtained was 0.00). Conclusions: It has been proved that\udusing sap of J. multifida has some effects on hemostasis, so its usage in traditional medicine is\udjustified. Moreover, it has no skin toxicity so its usage as local hemostatic is recommended