Open AccessIn Vitro Free Radical Scavenging Activity of Gallic Acid Isolated From Caesalpinia Decapetala Wood
Author(s) -
Upendra Bhadoriya,
Praveen Kumar Sharma,
Shailendra Singh Solanki
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60274-6
Subject(s) - gallic acid , chemistry , dpph , abts , caesalpinia , antioxidant , chromatography , ethanol , high performance liquid chromatography , traditional medicine , organic chemistry , medicine
Objective: Antioxidant capacities and phenolic contents of Gallic Acid isolated from plant\udCaesalpinia decapetala were investigated. Caesalpinia decapetala is a plant used to treat\udburns, biliousness and stomach disorders. Methods: The antioxidant activity was estimated by\udthe ABTS and DPPH methods. The total phenolic was measured using Folin-Ciocalteu assay.\udIdentification of isolated Gallic Acid was also performed by reverse-phase high-performance\udliquid chromatography (RP-HPLC) using Cosmosil C18 column (150mm x 4.6mm, 5毺 m particle).\udThe mobile phase was a mixture of ethyl acetate: ethanol: water (1:5:4, v/v/v) delivered at a flow\udrate of 1.0 mL min-1. Results: The total phenolic content was found to be 4.31% (w/w). Isolation of\udGallic Acid with optimum yield was performed using a mixture of solvents (Ethanol: Water 65:35).\udIsolated Gallic Acid showed significant in vitro free radical scavenging activity in both model; but\udin ABTS assay significant % inhibition of free radical was observed as compared to DPPH assay.\udConclusions: Research concluded that Caesalpinia decapetala extract can be used as potent\udantioxidant which can play vital role against the diseases like neurodegenerative disorders,\udinflammation, viral infections and gastric ulce