Open AccessAntioxidant and cytotoxic efficacy of chitosan on bladder cancer
Author(s) -
Senthilkumar Kuppusamy,
Jeyaprakash Karuppaiah
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60262-x
Subject(s) - chemistry , benzidine , glutathione peroxidase , antioxidant , glutathione , glutathione reductase , chitosan , pharmacology , lipid peroxidation , viability assay , biochemistry , superoxide dismutase , cell , medicine , enzyme
Objective: The present study demonstrated the antioxidant and cytotoxic efficacy of chitosan by\udevaluating cell viability in T24 human bladder cancer cell line and benzidine induced bladder\udcancer. The chemo preventive effects of the chitosan were evaluated in Swiss albino mice using\ud16 weeks medium term model of benzidine induced bladder cancer. Methods: Treatment of T24\udcells with increasing concentration of chitosan led to a concentration dependent decrease in cell\udmigration by MTT assay. The enzymic and non enzymic antioxidants were measured. Results:\udBladder cancer was induced twice weekly through oral incubation of benzidine for 4 weeks. The\udoral administration of chitosan (100mg KG-1 body wt) showed a significant increase in antioxidant\udenzymes like Super oxide dismutase (SOD), Glutathione peroxidase (GPx), Glutathione reductase\ud(GR), Catalase (CAT) and non-enzymic antioxidants like reduced Glutathione (GSH),vitamin C\udand vitamin E when compared to benzidine treated groups. The effect is more pronounced in\udpretreatment regime than in the post treatment regime. The levels of lipid peroxidation were\udsignificantly decreased in the chitosan treated regimes. Conclusions: The present study reveals\udthat chitosan has antioxidant and cytotoxic effects on benzidine induced bladder cancer and T24\udhuman bladder cancer cell line