Open AccessAntagonistic activity of marine sponge associated Streptomyces sp. against isolated fish pathogens
Author(s) -
G. Palani Selvan,
S. Ravikumar,
A. Ramu,
P. Neelakandan
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60252-7
Subject(s) - sponge , antibacterial activity , streptomyces , biology , microbiology and biotechnology , marine fish , accession number (library science) , strain (injury) , minimum inhibitory concentration , minimum bactericidal concentration , fish <actinopterygii> , veterinary medicine , bacteria , antimicrobial , botany , fishery , medicine , biochemistry , anatomy , genetics , genbank , gene
Objective: To investigate the antibacterial potential of the marine actinomycetes isolated from\udsponge samples. Methods: Thirty six marine sponge samples were collected from Palk Strait and\udfurther used for actinomycetes isolation by using serial dilution. The antibacterial activity was\udcarried out by using cross streak assay method. Moreover, most potential strain also subjected\udto MIC and MBC techniques and the isolated potential strain was identified by molecular tools.\udResults: The maximum counts (26 x 102 CFU/g) were observed in the month of May and minimum\udcounts (1 x 102 CFU/g) were noticed in April. A total of 21 actinomycetes were isolated and their\udantibacterial potential was assessed by using cross streak method. Among the 21 actinomycetes,\udthe ACT-21 showed sensitivity against all the isolated fish pathogens. Further, the MIC and MBC\udresults reveal that, the ACT-21 showed sensitivity at the concentration ranged between 500 毺 g/\udmL-1 500 毺 g/mL. The phylogenetic analysis suggested that, the potential isolate ACT-21 (accession\udno: JF899543) showed maximum similarity index (>98%) with Streptomyces sp. Conclusions: It is\udconcluded from present study that, the crude extracts of sponge associated actinomycetes could\udbe used as an effective antibacterial agent for the management of disease free fish culture system