Open AccessEvaluation of selected Indian traditional folk medicinal plants against Mycobacterium tuberculosis with antioxidant and cytotoxicity study
Author(s) -
K.V. Tawde,
Rajesh N. Gacche,
Mahesh Pund
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60244-8
Subject(s) - andrographis paniculata , traditional medicine , dpph , cytotoxicity , medicinal plants , mycobacterium tuberculosis , acanthaceae , chemistry , antioxidant , medicine , tuberculosis , biochemistry , in vitro , alternative medicine , pathology
Objective: To evaluate different solvent extracts of selected Indian traditional medicinal plant\udagainst Mycobacterium tuberculosis, its antioxidant potential and cytotoxicity. Methods: Acacia\udcatechu (L.) Willd (Root extract) and Ailanthus excelsa Roxb., leaf extracts of Aegle marmelos\udCorr., Andrographis paniculata Nees. and Datura metel L. were sequentially extracted in water,\udethanol, chloroform and hexane and evaluated for their anti-tuberculosis (TB) activity against\udMycobacterium tuberculosis using agar diffusion assay. The zone of inhibition ( at 20 and 40 mg/\udml) was measured and MIC were calculated. The results were compared with Rifampicin as a\udstandard anti TB drug. The extracts were also evaluated for DPPH and OH radical scavenging\udactivities to understand their antioxidant potential. MTT based cytotoxicity assay was used for\udevaluating cytotoxicity of the selected samples against Chang liver cells. Results: The selected\udbotanicals were sequentially extracted in water, ethanol, chloroform and hexane and tested for\udgrowth inhibition of M. tuberculosi. The hexane extract of A. catechu root and ethanol extract of\udA. paniculata leaf showed promising activity against M. tuberculosis while remaining extracts\udshowed moderate anti TB activity. The samples were found to possess considerable DPPH and\udOH radical scavenging activities with no demonstrable cytotoxicity against Chang liver cells.\udConclusions: Five traditional medicinal plants were selected for the present study. The selection\udof medicinal plants was based on their traditional usage for the treatment of tuberculosis, asthma\udand chronic respiratory diseases. Herein we report for the first time, the anti TB activity of root\udextracts of Acacia catechu and Ailanthus excelsa while leaf extract of Andrographis paniculata,\udAegle marmelos and Datura metel. The study holds importance in the midst of multi drug\udresistance (MDR) crisis in the TB management, since it unravels the scientific basis of use of these\udplant species for the management of TB and related disorders which will be useful for searching\udthe lead compounds from natural products as potential antimycobacterial agents