Open AccessDo diverse Mycobacterium tuberculosis strains circulate in Mumbai?
Author(s) -
Viral Vadwai,
Anjali Shetty,
Camilla Rodrigues
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60221-7
Subject(s) - typing , mycobacterium tuberculosis , biology , genetics , tuberculosis , tandem repeat , population , medicine , gene , genome , environmental health , pathology
Objective: To facilitate large network surveillance, we evaluated use of spoligotyping in\udcombination with different formats (12-, 15- and 24- loci) of to Mycobacterial Interspersed\udRepetitive Units (MIRU)-Variable Number Tandem Repeats (VNTR) achieve maximal\uddiscriminatory power. Methods: Spoligotyping and 24-loci MIRU-VNTR was performed on 127\udconsecutive extrapulmonary Mycobacterium tuberculosis (M. tuberculosis) isolates. Results: Based\udon the analysis of 127 M. tuberculosis isolates, all 3 formats of MIRU-VNTR when coupled with\udspoligotyping (HGDI: spoligotyping +12-loci, 0.9 967