Open AccessIn vitro Evaluation of Antimitotic, Antiproliferative, DNA fragmentation and Anticancer activity of Chloroform and Ethanol extracts of Revia hypocrateriformis
Author(s) -
Shweta Saboo,
S. S. Khadabadi,
Ganesh G. Tapadiya
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60211-4
Subject(s) - chemistry , petroleum ether , chloroform , mitotic index , cytotoxicity , ethanol , in vitro , pharmacology , traditional medicine , ic50 , dna fragmentation , mtt assay , biochemistry , chromatography , biology , mitosis , extraction (chemistry) , apoptosis , programmed cell death , medicine , microbiology and biotechnology
Objective: The plant Rivea hypocrateriformis (RH) has numerous therapeutic utility in folk\udmedicine having antidiabetic, antidepressant, analgesic as well as pregnancy irruption\udand anticancer properties. This led us to carry out the evaluation of plant for antimitotic,\udantiproliferative and cytotoxicity studies. Materials and Method: The dried aerial parts of RH\udwere successively extracted with petroleum ether, chloroform, ethanol and water. All extracts are\udsubjected to in vitro Antimitotic and Antiproliferative assay by Allium cepa root inhibition and\udyeast model. The successive chloroform, SCH and ethanol extract, SEE was subjected to in vitro\udanticancer activity by SRB assay MCF-7, HOP-62, MOLT-4, HCT-15 and PRO cell lines. Results:\udThe SCH and SEE shows significant antimitotic and antiproliferative activity. The mitotic index\udwas found to be 12.14 and 14.24 mg/mL respectively, which was near to standard, Methothrexate\ud11.39. The IC50 value of antiproliferative assay was found to be 47.88 to 27.12 mg/mL for SCH and\udSEE respectively. Conclusions: Based on these results, it is concluded that RH may be the good\udcandidate for the treatment of cancer as SCH and SEE are cytotoxic against various cell line in\udSRB assay