Open AccessNephroprotective effects of Colpomenia sinuosa (Derbes & Solier) against carbon tetrachloride induced kidney injury in Wistar rats
Author(s) -
Lekameera Ramarajan,
Somasundaram S. Thirugnanasambandan,
Sethupathy Subramanian,
P. Vijayabaskar
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60199-6
Subject(s) - ccl4 , carbon tetrachloride , chemistry , tbars , antioxidant , glutathione , albumin , oxidative stress , medicine , toxicity , creatinine , lipid peroxidation , pharmacology , endocrinology , biochemistry , biology , enzyme , organic chemistry
Objective: To establish the protective effect of seaweed Colpomenia sinuosa against carbon tetra\udchloride (CCl4) induced oxidative stress and resultant dysfunction of rat kidney. Methods: Seven\udto eight weeks old male Wistar rats (150-220g) were exposed to CCl4 (1.5 ml/kg) injection then\udtreated with seaweed Colpomenia sinuosa (100 mg/kg body weight in 0.3% CMC solution). Blood\udwas collected at the 5th day of experimental period to estimate the Total count (TC), Hemoglobin\ud(HB), Total protein (TP), Glucose, Albumin, Cholesterol, TGL and Urea. Results: The results shows\udsignificantly decreased (P<0.01) level of TC, the cholesterol and urea levels shows significantly\udincreased (P<0.05) in CCl4 treated groups when compared to control groups. These levels were\udfound to be normalized by oral feeding of C. sinuosa. Then the rats were sacrificed and kidneys\udtaken for enzyme analyses and histological examination. In the CCl4 treated group significantly\udincreased activities in TBARS, SOD, CAT, GPX, GSH (P<0.05) when compared to control group.\udThese increased activities were found to near normal in the CCl\ud4 + C. sinuosa treated group and\udSeaweed C. sinuosa treated alone group did not change any enzyme activity. Exposure to CCl4\udresulted hydrobhic changes in epithelium and Hypercellulartity of glomerulus was seen in the\udCCl\ud4 + drug treated group. Conclusions: These results suggest that the nephroprotective effect\udof C. sinuosa can be attributed to its enhancing effects on antioxidant defense system and lead to\udprevent the damage by exposure of CCl4 toxicity