Open AccessComparative evaluation of antioxidant and antimicrobial activity of crude extract and secondary metabolites isolated from Artemisia kulbadica
Author(s) -
Elham Ezzatzadeh,
Mohammad Hossein Farjam,
Abdolhossein Rustaiyan
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60198-4
Subject(s) - antimicrobial , dpph , antioxidant , chemistry , traditional medicine , agar dilution , food science , minimum inhibitory concentration , artemisia , biochemistry , organic chemistry , medicine
Objective: To investigate the antimicrobial and antioxidant properties of Et2O/MeOH/petrol extract\udand three isolated compounds from Artemisia kulbadica (A. kulbadica). Methods: The antimicrobial\udactivity was tested by using the disc-diffusion method and determining the minimal inhibitory\udconcentration (MIC) using the agar dilution method against Gram positive and Gram negative bacteria,\udand fungi. The antioxidant activities of crude extract and tree isolated compounds were evaluated\udusing 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. Results: The plant\udextract was showed moderate values DPPH radical scavenging activity (IC50= (422.4 暲 2.4) 毺 g/mL)\udwhile it was showed no considerable antimicrobial activity against tested microorganisms. Three\udisolated compounds tested for the first time, demonstrated antimicrobial and antioxidant activities.\udTwo sesquiterpenes showed higher antimicrobial activity than the flavone while the later compound\udwas better antioxidant than the sesquiterpens. Conclusions: The present study clearly demonstrated\udthat A. kulbadica and some of its isolates each one separately possess antimicrobial or antioxidant\udproperties and may act as potential antioxidant for biological systems susceptible to free radicalmediated reactions