Open AccessAntibacterial activity of honey alone and in combination with Nigella sativa seeds against Pseudomonas aeruginosa infection
Author(s) -
Abdelmalek Meslem,
Ahmed Moussa,
Noureddine Djebli,
Saâd Aissat
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60197-2
Subject(s) - nigella sativa , pseudomonas aeruginosa , agar dilution method , antibacterial activity , microbiology and biotechnology , agar , chemistry , traditional medicine , minimum inhibitory concentration , biology , food science , antimicrobial , bacteria , medicine , genetics
Objective: To evaluate the in vitro activities, of three honeys sample, and Nigella sativa (N.\udsativa) against Pseudomonas aeruginosa (P. aeruginosa) alone and in combination. Methods:\udThe antibacterial test and minimum inhibition concentration (MIC) was determined by using agar\udwell diffusion and dilution methods respectively against P. aeruginosa. Results: The MIC for the\udthree varieties of honey without N. sativa against P. aeruginosa ranged between 46% and 50% (v/v).\udAddition of N. sativa (8%) resulted in synergistic bactericidal activity. An MIC drop was noticed\udwith each variety and it ranged between 77.77% and 84.21%. Conclusions: These antibacterial\udproperties would warrant further studies on the clinical applications of N. sativa and honey\udagainst P. aeruginosa