Open AccessEvaluation of in vivo and in vitro anti-inflammatory activity of Ajuga bracteosa Wall ex Benth
Author(s) -
Raghunath Singh,
SK . Patil,
Gagandeep Pal,
Mumtaz Ahmad
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60191-1
Subject(s) - diclofenac sodium , ex vivo , chemistry , pharmacology , in vivo , carrageenan , anti inflammatory , traditional medicine , in vitro , medicine , biochemistry , biology , microbiology and biotechnology
Ethnopharmacological relevance: Ajuga bracteosa Wall Ex Benth. (Labiateae) is described in\udAyurveda for the treatment of rheumatism, gout, palsy and amenorrhea.Objective: Present study\udwas aimed to investigate the in vivo and in vitro anti-inflammatory activity of Neelkanthi (whole\udplant) and to support its traditional use.Methods: Methanolic extract of plant Ajuga bracteosa\ud(ABE) was investigated for its anti-inflammatory activity in carrageenan induced rat paw oedema,\udegg albumin induced inflammation in rats and the study was further supported with in vitro antiinflammatory study by using Human red blood cell membrane stabilization (HRBC) method. Three\uddoses of the extract (ABE- 250, 500 and 750 mg/kg, i.p.) were used in the study and diclofenac\udsodium (5mg/kg, i.p.) was used as standard. Results: ABE (500 and 750 mg/kg, i.p.) significantly\ud(P<0.05) reduced increased in paw volume induced by carrageenan and egg albumin. ABE also\udshowed significant stabilization toward HRBC membrane. Conclusions: ABE at the dose of 500\udand 750 mg/kg showed potent action on comparison with the standard drug diclofenac sodium