Open AccessSerological diagnosis for active tuberculosis in Malaysian population: Comparison of four protein candidate
Author(s) -
Siti Suraiya,
Mustaffa Musa,
Rapeah Suppian,
Jalaluddin Ashraful Haq
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60172-8
Subject(s) - serology , tuberculosis , medicine , immunology , pulmonary tuberculosis , population , active tuberculosis , mycobacterium tuberculosis , antibody , pathology , environmental health
Objective: To asses the ability of 4 types of Mtb proteins-ESAT6, SCWP, MAN and Ag85 to\udserve as indicator for active tuberculosis among Malaysian population. Methods: Sera from 90\udindividuals, 60 from confirmed pulmonary tuberculosis patients and 30 healthy PPD negative\udindividuals were tested for presence of anti-IgG and anti IgA by ELISA assay. Result: Mean\udconcentration of IgG and IgA were higher in patients compared to healthy Positivity of the ELISA\udtest were calculated, taking the cut off value at mean +2 SD of healthy sera. The sensitivity\udof the ELISA IgA assay for ESAT 6, SCWP, MAN and Ag85 were 81.1%, 83.3%, 11.7% and 53.3%\udrespectively. The sensitivity of the ELISA IgG asay for ESAT 6, SCWP, MAN and Ag85 were 71.0%,\ud71.0%, 71.0% and 21.0% respectively. Conclusion: Detectionof IgA against SCWP promised a good\udindicator for active tuberculosis infection among Malaysian