Open AccessEvaluation of antibacterial activity of selected medicinal plant extracts from south India against human pathogens
Author(s) -
Chinnaperumal Kamaraj,
Abdul Abdul Rahuman,
Siva Chidambaram,
Moorthy Iyappan,
Arivarasan Vishnu Kirthi
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60169-8
Subject(s) - gymnema sylvestre , enterobacter aerogenes , traditional medicine , ethyl acetate , bacillus cereus , cassia , antibacterial activity , minimum inhibitory concentration , cereus , biology , agar diffusion test , botany , escherichia coli , microbiology and biotechnology , bacteria , antimicrobial , medicine , biochemistry , genetics , alternative medicine , traditional chinese medicine , pathology , gene
Objective: The present study was to evaluate the antibacterial properties of 21 crude extracts\udfrom leaf and flower of Aristolochia indica (A. indica), Cassia angustifolia (C.angustifolia),\udleaf of Catharanthus roseus (C. roseus), Diospyros melanoxylon (D.melanoxylon), Dolichos\udbiflorus (D. biflorus), Gymnema sylvestre (G. sylvestre) and Justicia procumbens (J. procumbens).\udMethods: The ethyl acetate, acetone and methanol extract of medicinal plants were evaluated\udagainst Gram-positive Bacillus cereus (B. cereus) and Gram-negative bacteria Aeromonas\udhydrophila (A. hydrophila), Enterobacter aerogenes (E. aerogenes), Escherichia coli (E. coli)\udand Klebsiella pneumoniae (K. pneumoniae) by using well diffusion assay and minimum\udinhibitory concentration (MIC). Results: The crude plant extracts demonstrated broad spectrum\udactivity against all bacteria. The highest inhibitory zone was observed in leaf methanol extract\udof A. indica against E. aerogenes (25 mm), and E. coli (20 mm), flower methanol extract of C.\udangustifolia against B. cereus (22 mm) and leaf acetone extract of G. sylvestre against B. cereus\ud(22mm). The MIC values of leaf methanol extract of A. indica against K. pneumonia (22.6 毺 g/\udml), and flower extract showed against E. coli (MIC: 24.2毺 g/ml), leaf ethyl acetate extract of\udC. angustifolia against K. pneumoniae (MIC: 28.4 毺 g/ml). Acetone ethyl acetate and methanol\udextracts of D. melanoxylon and D. biflorus showed the lowest MIC activity value of >30 毺 g/ml\udagainst all tested pathogens. Conclusion: The antibacterial activity could be confirmed in most\udspecies used in traditional medicine in South India. Nevertheless, traditional knowledge might\udprovide some leads to elucidate potential candidates for future development of new antibiotic\udagents