Open AccessIn vitro antibacterial activity of various tissue types of Dumortiera hirsuta (Sw) Nees from different altitudes of eastern Himalaya
Author(s) -
Souryadeep Mukherjee,
Arijit De,
Pinky Ghosh,
Abhijit Dey
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60167-4
Subject(s) - thallus , antibacterial activity , bryophyte , pathogenic bacteria , biology , minimum inhibitory concentration , bacteria , altitude (triangle) , botany , antibiotics , traditional medicine , microbiology and biotechnology , medicine , genetics , geometry , mathematics
Objective: To study the antibacterial activity of methanol and ethanol extracts of vegetative, male\udsexual and female sexual reproductive thalli of the bryophyte Dumortiera hirsuta collected from\udfive different altitudes of eastern Himalaya against some human pathogenic bacteria. Methods:\udWell diffusion method, determination of minimum inhibitory concentration (MIC) and minimum\udbactericidal concentration (MBC) were carried out against strains of human pathogenic bacteria.\udResults: Different tissue types of the bryophyte collected from various altitudes have shown to\udinhibit the human pathogenic bacterial strains in vitro. Maximum antibacterial activity was noted\udagainst S. marcescens (ATCC 13880) for both the 80% methanolic and ethanolic extracts of both\udthe vegetative and the reproductive thalli. The extracts of the reproductive thallus showed less\udantibacterial activity than that of the vegetative thallus. Specimens collected from higher altitude\udshowed slightly lesser antibacterial activity than their lower altitude counterparts. Conclusions:\udAntibiotic activity was noted to be dependent on solvent system used for extraction, altitude and\udtissue type. Analyses of extracts may yield some bioactive molecule responsible for antibacterial\udefficacy of the bryophyte