Open AccessFabrication and Evaluation of Tinidazole Microbeads for Colon Targeting
Author(s) -
Amit Pandey,
Niharika Choudhary,
Vineet Kumar,
Harinath Dwivedi,
Koshy M. Kymonil,
Shubhini A. Saraf
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60151-0
Subject(s) - tinidazole , pectin , chemistry , calcium alginate , chromatography , sodium alginate , calcium , sodium , food science , biochemistry , organic chemistry , metronidazole , antibiotics
Objective: The purpose of present investigation was to develop and evaluate multiparticulate\udsystem exploiting pH-sensitive property and specific biodegradability of calcium alginate\udmicrobeads, for colon- targeted delivery of Tinidazole for the treatment of amoebic colitis.\udMethods: Calcium alginate beads containing Tinidazole were prepared by ionotropic gelation\udtechnique followed by coating with Eudragit S100 using solvent evaporation method to obtain\udpH sensitive microbeads. Various formulation parameters were optimized which included\udconcentration of sodium alginate (2% w/v), curing time (20 min) and concentration of pectin (1% w/\udv). All the formulations were evaluated for surface morphology, particle size analysis, entrapment\udefficiency and in-vitro drug release in conditions simulating colonic fluid in the presence of\udrat caecal (2% w/v) content. Results: The average size of beads of optimized formulation (FT4)\udwas found to be 998.73依5.12 毺 m with entrapment efficiency of 87.28依2.19 %. The invitro release\udof Eudragit S100 coated beads in presence of rat caecal content was found to be 70.73%依1.91%\udin 24 hours. Data of in-vitro release was fitted into Higuchi kinetics and Korsmeyer Peppas\udequation to explain release profile. The optimized formulation (FT4) showed zero order release.\udConclusions: It is concluded that calcium alginate microbeads are the potential system for\udcolon delivery of Tinidazole for chemotherapy of amoebic infection