Open AccessStandardization of Berberis aristata extract through conventional and modern HPTLC techniques
Author(s) -
Dinesh Kumar Patel,
Krutika Patel,
S. P. Dhanabal
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60139-x
Subject(s) - phytochemical , flavonoid , chemistry , tannin , traditional medicine , palmatine , berberine , antimicrobial , berberis , saponin , food science , biochemistry , medicine , organic chemistry , antioxidant , alternative medicine , pathology
Objective: Berberis aristata (Berberidaceae) is an important medicinal plant, found in the\uddifferent region of the world. It has significant medicinal value in the traditional Indian and\udChinese system of medicine. The aim of the present investigation includes qualitative and\udquantitative analysis of Berberis aristata extract. Methods: Present study includes determination\udof phytochemical analysis, solubility test, heavy metal analysis, antimicrobial study and\udquantitative analysis by HPTLC method. Results: Preliminary phytochemical analysis showed the\udpresence of carbohydrate, glycoside, alkaloid, protein, amino acid, saponin, tannin and flavonoid.\udSolubility in water and alcohal were found to be 81.90% in water and 84.52% in 50% in alcohal.\udLoss on drying was found to be 5.32%. Total phenol and flavonoid content were found to be 0.11%\udand 2.8%. Level of lead, arsenic, mercury and cadmium complies the standard level. E. coli and\udsalmonella was found to be absent whereas total bacterial count, yeast and moulds contents\udwere found to be under the limit. Content of berberine was found to be 13.47% through HPTLC\udtechniques. Conclusions: The results obtained from the present studies could be used as source\udof valuable information which can play an important role for the food scientists, researchers and\udeven the consumers for its standards