Open AccessAntimicrobial study of plant extracts of Datura metel L. against some important disease causing pathogens
Author(s) -
Varahalarao Vadlapudi,
D. S. V. G. K. Kaladhar
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60130-3
Subject(s) - antimicrobial , chloroform , agar diffusion test , chemistry , erwinia , chromatography , pseudomonas syringae , minimum inhibitory concentration , antibacterial activity , bacteria , microbiology and biotechnology , microorganism , traditional medicine , biology , medicine , genetics
Objective: In this present study antimicrobial activity of aerial parts of Datura metel L were\udevaluated against the resistant pathogens belong to aquatic, human and plant origin. Methods:\udSoxhlet extraction method was used to get the corresponding extracts of hexane, chloroform\udand methanol. The antimicrobial activities of the organic solvent extracts on the various test\udmicroorganisms, including bacteria and fungi investigated using agar well diffusion technique.\udThe length of inhibition zone was measured in millimeters from the edge of the well to the edge of\udthe inhibition zone. Methanol and chloroform extracts exhibited promising antimicrobial activity\udthan hexane extracts. Results: The zone of inhibition of chloroform varies from (9 to 18 mm)\udwhere as with methanol (11 to 30 mm) at 100 mg/ml and chloroform (11 to 19 mm) and methanol (12\udto 35 mm) with 250 mg/ml DMSO concentrations consequently. Among all microorganisms studied\udErwinia caratovara and Pseudomonas syringae showed the considerable growth inhibition with\udchloroform and methanolic extracts. Conclusions: D. metel can be used in the treatment of\udinfectious diseases caused by resistant pathogenic microorganisms. Further studies are being\udcarried out in order to separate the individual components that are present in plant extracts of D.\udmetel using column chromatography