Open AccessInvestigation on cytotoxic, antioxidant, antimicrobial and volatile profile of Wrightia tinctoria (Roxb.) R. Br. flower used in Indian medicine
Author(s) -
S Ramalakshmi,
N Edaydulla,
P. Ramesh,
K. Muthuchelian
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60126-1
Subject(s) - chemistry , dpph , antimicrobial , brine shrimp , phenols , flavonoid , antioxidant , traditional medicine , antibacterial activity , food science , carotenoid , biochemistry , biology , organic chemistry , bacteria , medicine , genetics
Objective: To investigate the total phenols, flavonoids, carotenoids, antioxidant activity,\udantimicrobial and cytotoxic activity of Wrightia tinctoria flower extract. Methods: Total\udphenols, flavonoids, carotenoids content, DPPH scavenging activity, the reducing power\udactivity, phosphomolybednum activity, metal chelating activity, Hydrogen peroxide radical\udscavenging activity of crude extract, Cytotoxicity activity, GC-MS analysis and Antibacterial\udscreening were evaluated. Results: Total phenols, flavonoids, carotenoids in the extract was\udfound to be 55.29±0.45 mg GAE, 370.53±1.213 mg QE and 1.825±0.321 mg/g respectively, where the\udreducing power, phosphomolybednum activity and metal chelating activity were increasing with\udincreasing concentration of the flower extract. The antioxidant activity (IC50) of the flower extract\udwas said to be 43.16 毺 g/mL by 2,2-Diphenyl-1-Picrylhydrazyl method and 124.07 mg AAE/100g\udof plant extract by phosphomolybednum method. The antibacterial studies of the ethanolic flower\udextract tested at different concentration of extracts, where 250mg/mL concentration of extract\udshowed good inhibitory activity against all the test pathogens compared with standard antibiotics\udlike streptomycin and penicillin. The cytotoxic activity of flower extract was evaluated by brine\udshrimp lethality bioassay method and the LC50 value found to be 3.544 毺 g/mL. Conclusions: The\udpresence of major bioactive compound, hexadecanoic acid justifies the use of the whole plant for\udvarious ailments by traditional practitioners. Further studies are needed to explore the potential\udphenolics, flavonoid compounds from W. tinctoria for application in drug delivery, nutritional or\udpharmaceutical fields