Open AccessCardioprotective effect of Spathodea campanulata bark on isoproterenol-induced myocardial infarction in rats
Author(s) -
Siddiq Abubaker,
I Shanmukha,
TM Jyoti,
Kamlesh Gupt
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60113-3
Subject(s) - lactate dehydrogenase , lipid peroxidation , thiobarbituric acid , creatine kinase , cardioprotection , glutathione , chemistry , tbars , myocardial infarction , antioxidant , creatine , isoprenaline , pharmacology , medicine , biochemistry , enzyme , stimulation
Objective: To evaluate the cardioprotective potential of 70% ethanolic extract of bark of Spathodea\udcampanulata P. Beauv (EEBSC). Methods: Wister strain rats were pretreated with EEBSC in two\uddifferent doses (250 and 500mg/kg) orally for 14 d and then intoxicated with isoproterenol (200 mg/\udkg, s.c. for 2 consecutive days at an interval of 24 h on 14th and 15th day of treatment protocol) to\udinduce myocardial injury. Results: Cardioprotection was assessed by estimating serum aspartate\udaminotransferase, alanine aminotransferase, lactate dehydrogenase, creatine phosphokinase,\udtriglycerides, total cholesterol, low density lipoproteins and high density lipoproteins. The levels of\udthiobarbituric acid reactive substances and reduced glutathione were analyzed in heart homogenate.\udIn isoproterenol-treated group, shrinkage of cardiac markers in serum and elevated lipid peroxidation\udaccompanied by decreased content of reduced glutathione in heart. The prior administration of\udEEBSC significantly (P<0.05) prevented the isoproterenol-induced alterations and restored the cardiac\udmarkers. The observed results were further confirmed by histopathological findings. Conclusions:\udThese findings indicate that EEBSC exerts cardioprotective effect against isoproterenol induced\udmyocardial infarction due to its free radical scavenging effects, which maintains the tissue defence\udsystem against myocardial damage