Open AccessRepellent activity of Ageratum houstonianum Mill. (Asteraceae) leaf extracts against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)
Author(s) -
Samuel Tennyson,
John Ravindran,
Alex Eapen,
John William
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60104-2
Subject(s) - anopheles stephensi , culex quinquefasciatus , aedes aegypti , ageratum conyzoides , biology , ethyl acetate , bioassay , traditional medicine , hexane , botany , toxicology , veterinary medicine , chemistry , larva , chromatography , weed , medicine , biochemistry , genetics
Objective: To determine the repellent activity of hexane, ethyl acetate and methanol crude\udextracts of Ageratum houstonianum (A. houstonianum) leaves against adult Anopheles stephensi\ud(An. stephensi), Aedes aegypti (Ae. aegypti) and Culex quinquefasciatus (Cx. quinquefasciatus)\udmosquitoes. Methods: Bioassay on laboratory reared Swiss albino mice by topical application at a\udfixed concentration of 0.01 per cent with coconut oil as a base. Results: Crude leaf extracts of A.\udhoustonianum in combination with coconut oil repelled vector mosquitoes. Maximum protection\udfor a period of 11.30 h was obtained against Cx. quinquefasciatus and An. stephensi when treated\udwith methanol and hexane extracts followed by 8.30 h against Ae. aegypti in methanol extract.\udAmongst the three extracts, methanol extract gave the maximum protection of 95.0% against Cx.\udquinquefasciatus. Hexane and ethyl acetate extracts gave a maximum of 93.4% protection against\udAn. stephensi. Conclusions: The crude extracts of A. houstonianum leaves in combination with\udcoconut oil showed repellent activity with repellent quotient ranging from 0.6 to 0.9