Open AccessOccurrence of white spot syndrome virus in shrimp culturing waters and its brunt in specific pathogen free Litopenaeus vannamei with particular allusion to molecular verdicts
Author(s) -
MA Badhul Haq,
V Prabhuraj,
R. Vignesh,
V. Sedhuraman,
M. Srinivasan,
T. Balasubramanian
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60095-4
Subject(s) - white spot syndrome , litopenaeus , basophilic , shrimp , biology , penaeus monodon , pathogen , virus , virology , penaeus , shellfish , specific pathogen free , prawn , microbiology and biotechnology , veterinary medicine , fishery , aquatic animal , fish <actinopterygii> , pathology , medicine
Objective: To detect the water samples and shrimp samples in white spot syndrome virus\ud(WSSV) affecting and non-affecting zone. Methods: A total of 12 samples specific pathogen free\udLitopenaeus vannamei (L. vannamei); adult shrimp and larvae were randomly collected. Their\udgenomic DNA was isolated and subjected to PCR. Histopathological identifications were carried\udout, and the hematopoietic tissues with basophilic intranuclear inclusion bodies characteristic\udwere observed in moderate WSSV infected L. vannamei. Results: The PCR analysis showed\udthe appearance of a prominent band from the PCR amplified product of WSSV-DNA at internal\udcontrol band 848 bp at non-infected areas. Although low infection positive bands (20 copies) were\udshown at 296 bp continued from initial stage of the infection region. On a moderate and ascetic\udlevel were observed as 650 bp and 910 bp (200 and 2 000 copies), during the severe out break\udperiods. The gill epithelial cells were edematous and nuclei were hypertrophied with basophilic\udinclusions, but no pathological changes or hypertrophied nuclei were observed in any of\udL.vannamei tissues in WSSV uninfected region. The Intranuclear inclusion bodies characteristics\udof high level of WSSV infection presented in the gill region. Conclusions: The present study\udis significant, which investigated the level of WSSV transmission from the infected tiger prawn\udP.monodon to SPFL. vannamei in the WSSV impact region of Tamil Nadu coastal waters