Open AccessIn vitro antibacterial potential of metal oxide nanoparticles against antibiotic resistant bacterial pathogens
Author(s) -
R. Gokulakrishnan,
S. Ravikumar,
J. Anandha Raj
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60089-9
Subject(s) - streptococcus pneumoniae , klebsiella pneumoniae , microbiology and biotechnology , pseudomonas aeruginosa , staphylococcus aureus , antibacterial activity , antibiotics , chemistry , minimum bactericidal concentration , nanoparticle , in vitro , bacteria , in vivo , bacterial growth , streptococcus , minimum inhibitory concentration , antibiotic sensitivity , escherichia coli , biology , materials science , nanotechnology , biochemistry , genetics , gene
Objective: To investigate the antibacterial potential of 5 different metal oxide nanoparticles\udagainst antibiotic resistant bacterial pathogens viz., Pseudomonas aeruginosa, Klebsiella\udsp. Streptococcus pneumoniae, Staphylococcus aureus and Streptococcus sp. Methods: The\udantibacterial activity of the five different nanoparticles was assessed by well diffusion method.\udDifferent concentrations of the nanoparticles were analyzed by MIC and MBC techniques. Finally\udthe potential MgO nanoparticle was also subjected for the time kill assay method. Results: The\udresults reveal that, the MgO nanoparticle showed maximum sensitivity [(16.00依0.53) mm dia]\udagainst Streptococcus pneumoniae and showed minimum sensitivity against Klebsiella sp. [(9.00依\ud0.31) mm dia]. None of the nanoparticles showed sensitivity against the Streptococcus sp. The MIC\udresult reveals that, the MgO nanoparticle showed maximum inhibition at a concentration of 10 毺 g\udagainst Streptococcus pneumoniae. Moreover, the time kill assay reveals that, the bacterial growth\udwas inhibited from the 2nd h onwards at a concentration of 10毺 g. Conclusions: It is concluded\udfrom the present findings that, the MgO nanoparticle could be used as an alternative antibacterial\udagent after completing successful in vivo trials