Open AccessAntibacterial activity of various honey types of Algeria against Pathogenic Gram–Negative Bacilli: Escherichia coli and Pseudomonas aeruginosa
Author(s) -
Ahmed M. Mustafa,
Noureddine Djebli,
Meslem Abdelmelek,
Saâd Aissat
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60048-6
Subject(s) - pseudomonas aeruginosa , bacilli , minimum inhibitory concentration , escherichia coli , microbiology and biotechnology , serial dilution , antibacterial activity , agar diffusion test , gram , bacteria , chemistry , biology , antimicrobial , medicine , biochemistry , genetics , alternative medicine , pathology , gene
Objective: To assess the in vitro antibacterial activity of different honey types in Algeria on Gram\udnegative organismes. Methods: Different concentrations (10, 30, 50, 70, 100 % v/v) of honey were\udstudied in vitro using Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa).\udBriefly, two-fold dilutions of honey solutions were tested to determine the minimum inhibitory\udconcentration (MIC) against each type of microorganism, followed by more assays within a\udnarrower dilution range to obtain more precise MIC values. MIC was determined by both visual\udinspection and spectrophotometric assay at 620 nm. The antibacterial activity of these honey\udsamples was determined by the disc and well diffusion method. Results: The zone diameter\udof inhibition of honey for P. aeruginosa and E. coli was 0-30 and 0-38 mm, respectively, while\udthe MIC ranged 90-91% and 56-96%, respectively. Conclusions: The results show that Algerian\udhoneys possess antibacterial activity against Gram negative bacilli, and it can be developed into\udantibacterial agents