Open AccessMosquito larvicidal and biting deterrency activity of bud of Polianthes tuberosa plants extract against Anopheles stephensi and Culex quinquefasciatus
Author(s) -
Anjali Rawani,
Banerjee Atanu,
Goutam Chandra
Publication year - 2012
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(12)60046-2
Subject(s) - culex quinquefasciatus , anopheles stephensi , polianthes tuberosa , chloroform , larvicide , traditional medicine , biology , ethyl acetate , toxicology , chemistry , botany , larva , aedes aegypti , chromatography , biochemistry , medicine
Objective: To evaluate the larvicide and biting deterrency activity of bud of Polianthes tuberosa (P.\udtuberosa) against Culex quinquefasciatus (Cx. quinquefasciatus) and Anopheles stephensi (An. stephensi).\udMethods: Crude and solvent extract [ethyl acetate, chloroform: methanol (1:1, v/v), acetone] of fresh,\udmature, bud of P. tuberosa was tested against Cx. quinquefasciatus and An. stephensi. The repellent\udactivity tested by chloroform: methanol (1:1, v/v) solvent extract against both mosquito species. The\udappropriate lethal concentrations at 24 h for chloroform: methanol (1:1, v/v) extract was also studied\udon non target organisms such as Toxorhynchites larvae, Diplonychus annulatum and Chironomus\udcircumdatus. Results: In a 72 h bioassay experiment, 0.5 % crude extract showed the highest mortality\udand chloroform: methanol (1:1, v/v) solvent extract showed the highest mortality, the maximum (P <\ud0.05) mortality was recorded at a concentration of 60 mg/L. The chloroform: methanol (1:1, v/v) solvent\udextract provide 4 h protection against Cx. quinquefasciatus and 5 h against An. stephensi from biting.\udConclusions: Both crude and chloroform: methanol (1:1, v/v) extract showed efficient activity against\udCx. quinquefasciatus, so it could be used as a mosquito larvicide agent. There is no change in the\udactivity of non-target organism so, it is safe to use