Open AccessFirst report of natural infection of phlebotomines for Leishmania (Leishmania) chagasi captured in Ponta Porã, on the border between Brazil and Paraguay
Author(s) -
Ana Rachel Oliveira de Andrade,
Maria Elizabeth Moraes Cavalheiros Dorval,
Sônia Maria Oliveira de Andrade,
Alisson Marques,
Manoel Sebastião da Costa Lima-Júnior,
Baldomero Antônio Kato da Silva,
Renato Andreotti
Publication year - 2011
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(11)60060-1
Subject(s) - leishmania , leishmania chagasi , sandfly , visceral leishmaniasis , polymerase chain reaction , leishmaniasis , virology , biology , transmission (telecommunications) , nested polymerase chain reaction , veterinary medicine , parasite hosting , immunology , medicine , gene , genetics , electrical engineering , world wide web , computer science , engineering
Objective: Visceral leishmaniasis has been reported in all Brazilian regions and, in Mato Grosso\uddo Sul State, the occurrence of cases has increased significantly. The objective of this study was\udto identify the natural infection of phlebotomines by Leishmania in Ponta Porã, a Brazilian county\udbordering Paraguay. Methods: By using light CDC and Shannon traps, 185 sandfly females were\udcaptured, dissected and arranged in 107 pools subjected to DNA extraction and amplification by\udPolymerase chain reaction. Results: From the samples subjected to amplification, the fragment of\ud120 bp characteristic of Leishmania sp was observed in one of the groups, which is composed by\uda female species of Evandromyia cortelezzii (Brèthes, 1923). Conclusions: The minimum infection\udrate calculated for Ponta Porã was 0.54%, and the occurrence of flagellates and the confirmation\udof natural infection by Leishmania chagasi pose a serious concern for the transmission of visceral\udleishmaniasis in the region