Open AccessFast track method for the identification of multi–drug resistant tuberculosis on direct clinical specimen using combined drug media
Author(s) -
Ranjani Ramachandran,
Rajagopalan Lakshmi,
Kumar D Ravi,
K. Devika,
Fathima Rahman,
Wares Df
Publication year - 2011
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(11)60011-x
Subject(s) - drug , rifampicin , isoniazid , mycobacterium tuberculosis , tuberculosis , medicine , drug resistance , concordance , pharmacology , microbiology and biotechnology , biology , pathology
Objective: To combine isoniazid (INH) and rifampicin (RIF) in a single media to detect and\udevaluate multi-drug resistant Mycobacterium tuberculosis (MDR-TB) strains using clinical\udspecimens by direct and indirect drug susceptibility testing (DST). Methods: Drug susceptibility\udtesting for INH and RIF using individual and combined drug media was performed by minimum\udinhibitory concentration (MIC) method on direct clinical specimens. Results: The combined drug\udmedia showed complete concordance with individual drug media in the detection of MDR-TB\udby direct DST method and 89% efficiency with indirect DST method. Susceptibility results were\udavailable by 3 weeks after the receipt of clinical specimen using direct DST on combined drug\udmedia. Conclusions: Combined drug media can be used as a fast track method in large scale\udstudies warranting detection of MDR-TB