Open AccessDevelopment of bioanalytical parameters for standardization of Terminalia arjuna
Author(s) -
Dinesh Kumar Patel,
Krutika Patel,
S. P. Dhanabal
Publication year - 2013
Publication title -
journal of acute disease
Language(s) - English
Resource type - Journals
eISSN - 2589-5516
pISSN - 2221-6189
DOI - 10.1016/s2221-6189(13)60145-6
Subject(s) - terminalia arjuna , tannin , phytochemical , flavonoid , rutin , chromatography , saponin , traditional medicine , bioanalysis , medicine , food science , chemistry , biochemistry , terminalia , antioxidant , alternative medicine , pathology
Objective: To develop a noval bioanalytical parameters for standardization of Terminalia arjuna (T. arjuna) extract.Methods: In the present investigation, parameters such as preliminary phytochemical analysis, total phenol, flavonoid, tannin content, solubility test, heavy metal analysis, antimicrobial study and quantitative analysis by HPTLC method were performed in order to standardization.Results: Preliminary phytochemical analysis showed the presence of alkaloid, carbohydrate, tannin, steroid, triterpenoid, glycoside, saponin, flavonoid, amino acid and protein. Loss on drying and solubily in water was found to be 5.04% and 81.10%. Total flavonoid and phenol content was found to be 10.4% and 0.44%. Total tannin content was found to be 24.8%. The content of quercetin and rutin in T. arjuna was found to be 1.08%w/w and 0.16%w/w respectively. Further the level of heavy metal and microrganism were found to be under the limit.Conclusions: These bioanalytical parameters can be used as an important tool for the food scientists, researchers and even the consumers for its standards