Open AccessFormulation and in-vitro preliminary screening of polyphyto antilithiatic combination
Author(s) -
Abhishek Bharadwaj,
Kumud Upadhayaya,
N. V. Satheesh Madhav
Publication year - 2013
Publication title -
journal of acute disease
Language(s) - English
Resource type - Journals
eISSN - 2589-5516
pISSN - 2221-6189
DOI - 10.1016/s2221-6189(13)60129-8
Subject(s) - medicine , oxalate , calcium oxalate , calcium , in vitro , aqueous solution , urinary system , nuclear chemistry , drug , tris , incubation , chromatography , pharmacology , biochemistry , inorganic chemistry , organic chemistry , chemistry
Objective: To study people suffered from urinary stone problem by calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) containing stones.Methods: In the present study, CO crystals were grown by mixing and stirring two equal volumes of solutions A and B. Two solutions of following composition were mixed: A: Na2C2O4(2 mmol) and B: CaCl2·2H2O (10 mmol). The crystals after incubation in tris buffer were then subjected to the aqueous extract of the polyherbal formulation and cystone was taken as standard.Results: After the treatment with the extract the crystals were microscopically examined and elemental analysis for calcium was carried out. The formulation proved out to be very effective against the CO crystals.Conclusions: The results have come out to be very motivating and further pharmacological study could be carried out on the samples to reveal an effective drug for the urolithiasis. Data reveals that phytotherapeutic agents could be useful as either an alternative or a complementary therapy in the management of urolithiasis