Open AccessDevelopment of bioanalytical parameters for the standardization of Zingiber officinale
Author(s) -
Dinesh Kumar Patel,
S. P. Dhanabal
Publication year - 2013
Publication title -
journal of acute disease
Language(s) - English
Resource type - Journals
eISSN - 2589-5516
pISSN - 2221-6189
DOI - 10.1016/s2221-6189(13)60113-4
Subject(s) - rutin , phytochemical , chromatography , flavonoid , zingiber officinale , tannin , traditional medicine , quercetin , glycoside , saponin , medicine , chemistry , biochemistry , food science , stereochemistry , antioxidant , alternative medicine , pathology
ObjectiveTo develop noval bioanalytical parameters for standardization of Zingiber officinale (Z. officinale) extract.MethodsPhytochemical analysis, solubility test, heavy metal analysis, antimicrobial study and fingerprint analysis through HPTLC method were carried out in the present investigation. Moreover quantitative analysis of rutin and quercetin in Z. officinale extract through HPTLC techniques were also performed in the present investigation.ResultsPhytochemical analysis showed the presence of alkaloid, carbohydrate, tannin, steroid, triterpenoid, glycoside, triterpenoid, saponin, flavonoid, amino acid and protein. The total phenol and flavonoid content were found to be 0.59% and 4.50%. For quantitative analysis through HPTLC techniques, optimization of solvent system was done and the content of quercetin and rutin in the extract was found to be 3.22% (w/w) and 0.65% (w/w) respectively.ConclusionsThese above mentioned parameters can be used as an important tool for the standardization of Z. officinale extract