Open AccessEvaluation of glucose utilization capacity of bioactivity guided fractions of Hybanthus enneaspermus and Pedalium murex in isolated rat hemidiaphragm
Author(s) -
Dinesh Kumar Patel,
Sairam Krishnamurthy,
S Hemalatha
Publication year - 2013
Publication title -
journal of acute disease
Language(s) - English
Resource type - Journals
eISSN - 2589-5516
pISSN - 2221-6189
DOI - 10.1016/s2221-6189(13)60091-8
Subject(s) - fraction (chemistry) , in vitro , glucose uptake , chloroform , medicine , chromatography , biochemistry , chemistry , insulin , endocrinology
ObjectiveTo investigate glucose utilization capacity of bioactivity guided fractions of Hybanthus enneaspermus (H. enneaspermus) and Pedalium murex (P. murex) in isolated rat hemidiaphragm.MethodsDried coarsely powdered plant material was extracted in ethanol using soxhlation technique, further extract was fractionated using solvents of varying polarity. Glucose utilization capacity of bioactivity guided fractions using isolated rat hemidiaphragm was performed in the present study.ResultsThe entire tested fraction showed increased glucose uptake capacity, and was found to be maximum in case of chloroform fraction of P. murex extract (CHPM) which was quite comparable to standard insulin (P<0.05).ConclusionsIn vitro glucose uptake by hemidiaphragm study showed increased utilization of the glucose by hemidiaphragm in the presence of different fractions. From these findings we can conclude that that different fraction of both plant materials had some extra pancreatic mechanism like glucose uptake by peripheral tissues