Open AccessAnticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells
Author(s) -
Maram Shalabi,
Kh. Khilo,
Mahmoud M. Zakaria,
Mahmoud El-Sebaei,
Walied Abdo,
Walaa Awadin
Publication year - 2015
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/s2221-1691(15)30372-5
Subject(s) - aloe vera , traditional medicine , hepatocellular carcinoma , medicine , medicinal plants , hepatic carcinoma , biology
ObjectiveTo investigate the in vitro anticancer effect of Aloe vera (A. vera) and Calligonum comosum (C. comosum) extracts against hepatocellular carcinoma (HepG2) cells.MethodsHepG2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic (Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG2 cells were observed using light microscopy.ResultsThe IC50 values of A. vera and C. comosum extracts were (10.45 ± 0.31) and (9.60 ± 0.01) µg/mL respectively. The extracts separately increased cytotoxicity against HepG2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions.ConclusionsThe results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis