Open AccessProtective effect of Tetracera scandens L. leaf extract against CCl4-induced acute liver injury in rats
Author(s) -
Tung Bui Thanh,
Hải Nguyễn Thanh,
Hue Pham Thi Minh,
Huong Le-Thi-Thu,
Huong Duong Thi Ly,
Loi Vu Duc
Publication year - 2015
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/s2221-1691(15)30009-5
Subject(s) - superoxide dismutase , catalase , lipid peroxidation , glutathione peroxidase , antioxidant , carbon tetrachloride , ccl4 , chemistry , pharmacology , glutathione , oxidative stress , liver injury , biochemistry , medicine , enzyme , organic chemistry
Objective: To investigate the protective potential of ethanolic extracts of Tetracera scandens L. (T. scandens) against CCl4 induced oxidative stress in liver tissues.Methods: Dried leaf powder of T. scandens was extracted with ethanol and concentrated to yield a dry residue. Rats were administered with 100 mg/kg of ethanolic extracts orally once daily for one week. Animals were subsequently administered with a single dose of CCl4 (1 mL/kg body weight, intraperitoneal injection). Various assays, such as serum levels of alanine aminotransferase, aspartate aminotransferase, lipid peroxidation, protein oxidation (carbonyl protein group), tumor necrosis factor alpha, catalase, superoxide dismutase, and glutathione peroxidase, were used to assess damage caused by CCl4 and the protective effects of the ethanol extract on liver tissues.Results: Hepatotoxicity induced by CCl4 was evidenced by a significant increase in serum aspartate aminotransferase and alanine aminotransferase level, lipid peroxidation, protein carbonyl group, and tumor necrosis factor alpha, as well as decreased activity of the hepatic antioxidant enzymes (catalase, superoxide dismutase, and glutathione peroxidase). Treatment with ethanolic T. scandens extracts prevented all of these typically observed changes in CCl4-treated rats.Conclusions: Our findings indicate that T. scandens has a significant protective effect against CCl4 induced hepatotoxicity in rat, which may be due to its antioxidant properties