Open AccessMolecular characterization of Cryptosporidium xiaoi in goat kids in Bangladesh by nested PCR amplification of 18S rRNA gene
Author(s) -
Amam Zonaed Siddiki,
Sohana Akter Mina,
Zinat Farzana,
Bibi Ayesa,
Rasel Das,
Mohammad Alamgir Hossain
Publication year - 2015
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/s2221-1691(15)30007-1
Subject(s) - cryptosporidium , nested polymerase chain reaction , biology , feces , 18s ribosomal rna , phylogenetic tree , polymerase chain reaction , veterinary medicine , genetic diversity , microbiology and biotechnology , sequence analysis , gene , genetics , population , medicine , environmental health
ObjectiveTo investigate the prevalence of Cryptosporidium spp. in goat kids in selected areas of Bangladesh and to elucidate the potential zoonotic hazards.MethodsIn the present study, we have used Ziehl-Neelsen staining and nested PCR approach to identify and characterize the Cryptosporidium sp. from diarrhoeic feces of goat kids. A total of 100 diarrhoeic feces samples were collected from Chittagong region in Southern Bangladesh. For nested PCR analysis, specific primers for amplification of 581 base pair fragments of 18S rRNA gene were used.ResultsA total of 15% and 3% samples were found positive in microscopic study and in nested PCR analysis respectively. Phylogenetic analysis of sequence data showed similarity with that of Cryptosporidium xiaoi recorded from sheep and goat.ConclusionsTo our knowledge, this is the first report of Cryptosporidium xiaoi responsible for diarrhoea in goat kids in Bangladesh. Further study can highlight their zoonotic significance along with genetic diversity in other host species inside the country