Open AccessPengawetan Spermatozoa Menggunakan Metode Pengeringbekuan
Author(s) -
Takdir Saili,
Mulyoto Pangestu,
Mohamad Agus Setiadi,
Srihadi Agungpriyono,
Mozes R. Toelihere,
Arief Boediono
Publication year - 2005
Publication title -
hayati journal of biosciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.305
H-Index - 17
eISSN - 2086-4094
pISSN - 1978-3019
DOI - 10.1016/s1978-3019(16)30322-9
Subject(s) - spermatozoon , sperm , human fertilization , intracytoplasmic sperm injection , andrology , cryopreservation , semen , zona pellucida , biology , oocyte , sperm motility , in vitro fertilisation , anatomy , embryo , microbiology and biotechnology , medicine
Since the discovery of cryopreservation method for bull semen, cryopreservation become an alternative method for maintaining gamet resources of certain animal which is threatened or near extinction. This technology was then applied to the preservation of embryo, oocyte, ovary and testis. The application of intracytoplasmic sperm injection (ICSI) for which sperm motility is unnecessary had supported the effort to create simplified method such as freeze-drying for sperm preservation. Due to the benefit of ICSI over the conventional in vitro fertilization (IVF) the spermatozoon could be mechanically driven to pass through the zona pellucida and entering the cytoplasm of oocytes prior to fertilization. The freeze-drying method is an alternative method in sperm preservation which ignored the motility of sperm. The sperm resulted from this technique is in drying state, therefore, it might be stored in room temperature or in refrigerator. Many reports have claimed that freeze-dried sperm which is not motile but has an intact DNA was able to fertilize oocytes, even produced offspring in mouse