Open AccessIdentification of a Mimotope Peptide Bound to the SARS-CoV Spike Protein Specific Monoclonal Antibody 2C5 with Phage-displayed Peptide Library
Author(s) -
Rong-Hong Hua,
Donglai Wu,
Guangzhi Tong,
Yunfeng Wang,
Zhen Tian,
Yanjun Zhou
Publication year - 2006
Publication title -
chinese journal of biotechnology/shengwu gongcheng xuebao
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.153
H-Index - 19
eISSN - 1872-2075
pISSN - 1000-3061
DOI - 10.1016/s1872-2075(06)60051-4
Subject(s) - panning (audio) , monoclonal antibody , phage display , mimotope , epitope , peptide , peptide library , biology , microbiology and biotechnology , antibody , virology , peptide sequence , gene , biochemistry , genetics , zoom , lens (geology) , paleontology
To identify the epitope of SARS-CoV spike protein specific neutralizing monoclonal antibody (MAb) 2C5. The antibody was used as target and three rounds of bio-panning were conducted with phage-display peptide library. After the third panning, 20 phage-plague clones were randomly picked and analyzed for the binding ability with the MAb 2C5 by ELISA. The display sequence analysis demonstrated that among the twenty phage clones, eight clones displayed the same seven-peptide TPEQQFT. All these eight phage-clones showed strongest binding activity with 2C5 in phage ELISA analysis. Furthermore, phages displaying peptide TPEQQFT could specifically inhibit the binding of MAb 2C5 with SARS-CoV spike protein. The results demonstrated that TPEQQFT is a mimic epitope peptide containing neutralizing MAb 2C5. This study may provide information for further structural and functional analysis of spike protein and development vaccine for severe acute respiratory syndrome.