Open AccessStudy of Fusion Protein and Attachment Glycoprotein of Nipah Virus Expressed in Recombinant Baculovirus
Author(s) -
Xijun Wang,
Sen Hu,
Jinying Ge,
Qinghua Wang,
Liting Qin,
Zhigao Bu
Publication year - 2006
Publication title -
chinese journal of biotechnology/shengwu gongcheng xuebao
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.153
H-Index - 19
eISSN - 1872-2075
pISSN - 1000-3061
DOI - 10.1016/s1872-2075(06)60038-1
Subject(s) - recombinant dna , virology , vesicular stomatitis virus , western blot , sf9 , glycoprotein , fusion protein , antibody , virus , mononegavirales , rabies virus , microbiology and biotechnology , immunofluorescence , biology , antigen , paramyxoviridae , gene , immunology , viral disease , biochemistry , spodoptera
In this study, Recombinant baculoviruses rBac-NF and rBac-NG were generated for expressing F and G proteins Nipah virus (NiV) . The expression of recommbinant G (rNG) and F (rNF) protein in rBac-NF and rBac-NG infected cells were confirmed by western-blot. Both rNG and rNF showed sensitive and specific antigenic reaction to rabbit serum anti-Nipah virus in indirect immunofluorescence detection and indirect ELISA. Immunization with rBac-NF and rBac-NG infected insect cells elicited G ad F protein specific antibody responses in mice. Furthermore, the G ad F specific antibodies could neutralize the infectivity of the VSVdeltaG* F/G, the NiV F and G envelope glycoproteins psudotyped recombinant Vesicular Stomatitis Virus expressing green fluorescence protein. The results demonstrated F and G protein expressed by the recombinant baculoviruses could be safe economic diagnostic antigens for the surveillance and monitoring of NiV and promising subunit vaccines for the prevention of NiV.