Open AccessToona Sinensis Extracts Induced Cell Cycle Arrest and Apoptosis in the Human Lung Large Cell Carcinoma
Author(s) -
Wang ChengYuan,
Lin KaiHuang,
Yang ChihJen,
Tsai JongRung,
Hung JenYu,
Wang PeiHui,
Hsu HsengKuang,
Huang MingShyan
Publication year - 2010
Publication title -
the kaohsiung journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.439
H-Index - 36
eISSN - 2410-8650
pISSN - 1607-551X
DOI - 10.1016/s1607-551x(10)70010-3
Subject(s) - cell cycle , apoptosis , flow cytometry , cell cycle checkpoint , viability assay , cyclin d1 , microbiology and biotechnology , medicine , cell growth , western blot , cancer research , cell , biology , biochemistry , gene
Toona sinensis extracts have been shown to exhibit anti‐cancer effects in human ovarian cancer cell lines, human promyelocytic leukemia cells and human lung adenocarcinoma. Its safety has also been confirmed in animal studies. However, its anti‐cancer properties in human lung large cell carcinoma have not been studied. Here, we used a powder obtained by freeze‐drying the super‐natant of centrifuged crude extract from Toona sinensis leaves (TSL‐1) to treat the human lung carcinoma cell line H661. Cell viability was evaluated by the 3‐(4‐,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide assay. Flow cytometry analysis revealed that TSL‐1 blocked H661 cell cycle progression. Western blot analysis showed decreased expression of cell cycle proteins that promote cell cycle progression, including cyclin‐dependent kinase 4 and cyclin D1, and increased the expression of proteins that inhibit cell cycle progression, including p27. Furthermore, flow cytometry analysis showed that TSL‐1 induced H661 cell apoptosis. Western blot analysis showed that TSL‐1 reduced the expression of the anti‐apoptotic protein B‐cell lymphoma 2, and degraded the DNA repair protein, poly(ADP‐ribose) polymerase. TSL‐1 shows potential as a novel therapeutic agent or for use as an adjuvant for treating human lung large cell carcinoma.