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Analysis of altered genomic expression profiles in the senescent and diseased myocardium using cDNA microarrays
Author(s) -
Juhasz Ondrej,
Zhu Yi,
Garg Rahul,
Anisimov Sergey V.,
Boheler Kenneth R.
Publication year - 2002
Publication title -
european journal of heart failure
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.149
H-Index - 133
eISSN - 1879-0844
pISSN - 1388-9842
DOI - 10.1016/s1388-9842(02)00169-1
Subject(s) - transcriptome , dna microarray , gene expression , gene expression profiling , computational biology , biology , microarray , gene , complementary dna , genetics , function (biology) , population , genome , bioinformatics , medicine , environmental health
Cardiac function deteriorates with aging or disease. Short term, any changes in heart function may be beneficial, but long term the alterations are often detrimental. At a molecular level, functional adaptations involve quantitative and qualitative changes in gene expression. Analysis of all the RNA transcripts present in a cell's population (transcriptome) offers unprecedented opportunities to map these transitions. Microarrays (chips), capable of evaluating thousands of transcripts in one assay, are ideal for transcriptome analyses. Gene expression profiling provides information about the dynamics of total genome expression in response to environmental changes and may point to candidate genes responsible for the cascade of events that result in disease or are a consequence of aging. The aim of this review is to describe how comparisons of cellular transcriptomes by cDNA array based techniques provide information about the dynamics of total gene expression, and how the results can be applied to the study of cardiovascular disease and aging.