Premium
Down‐regulated expression of family with sequence similarity 3, member B (FAM3B), in oral squamous cell carcinoma
Author(s) -
Shiiba Masashi,
Ishige Shunsaku,
Saito Yasuhiro,
Shimizub Toshihiro,
Minakawa Yasuyuki,
Kasamatsu Atsushi,
Ogawara Katsunori,
Uzawa Katsuhiro,
Tanzawa Hideki
Publication year - 2012
Publication title -
oral science international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.256
H-Index - 13
eISSN - 1881-4204
pISSN - 1348-8643
DOI - 10.1016/s1348-8643(12)00004-3
Subject(s) - biology , microrna , carcinogenesis , microbiology and biotechnology , cancer research , cell culture , microarray analysis techniques , apoptosis , cancer , gene expression , gene , genetics
Purpose: Family with sequence similarity 3, member B (FAM3B), also known as PANcreatic DERived factor (PANDER), is an islet‐specific cytokine predominantly expressed in both pancreatic α‐ and β‐cells. FAM3B is known to induce apoptosis in pancreatic cells, and may therefore be able to regulate apoptosis in other cell types, including cancer cells. However, the role of FAM3B in carcinogenesis is unknown. We previously performed global gene screening using DNA microarray analysis to identify crucial molecules in oral squamous cell carcinoma (OSCC). The results showed significant down‐regulation of FAM3B in OSCC‐derived cell lines, as compared with human normal oral keratinocytes (HNOKs). The aim of the present study was to clarify the association between FAM3B and OSCCs. Results: Expression profiles of FAM3B in OSCC‐derived cell lines and human primary OSCCs were examined by real‐time quantitative reverse transcriptase‐polymerase chain reaction and Western blot analysis. Significantly decreased expression of FAM3B mRNA and FAM3B protein were observed in five OSCC‐derived cell lines, as compared with HNOKs. Moreover, immunohistochemical analysis revealed down‐regulated FAM3B protein expression in primary OSCC samples. In order to examine whether microRNA (miRNA) potentially regulates FAM3B expression, miRNA microarray analysis was performed with OSCC‐derived cell lines. miRNA microarray data showed that miR‐181a, miR‐181b, miR‐200b, and miR‐200c, which can induce imperfect base pairing with the 3′‐untranslated region of FAM3B mRNA, were up‐regulated in all cell lines examined. Conclusions: These results indicate that decreased FAM3B expression is able to promote OSCC progression and that certain miRNAs may be correlated with the altered expression of FAM3B .