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Effects of Heparin‐binding Epidermal Growth Factor on the Invasion Activity of an Oral Cancer Cell Line
Author(s) -
Furukawa Masayuki,
Ohnishi Yuichi,
Kakudo Kenji
Publication year - 2008
Publication title -
oral science international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.256
H-Index - 13
eISSN - 1881-4204
pISSN - 1348-8643
DOI - 10.1016/s1348-8643(08)80004-3
Subject(s) - epidermal growth factor , transfection , small interfering rna , autocrine signalling , cell culture , matrigel , paracrine signalling , biology , growth factor , microbiology and biotechnology , cancer research , cell growth , a431 cells , chemistry , cell , oncogene , cell cycle , angiogenesis , biochemistry , receptor , genetics
Heparin‐binding epidermal growth factor (EGF)‐like growth factor (HB‐EGF), which belongs to the EGF family, has been shown to stimulate the growth of a variety of cells in an autocrine or paracrine manner. Although HB‐EGF is widely expressed in tumors when compared to normal tissue, its contribution to tumor invasion is still not known. In the present study, we analyzed the effects of HB‐EGF on the invasion activity of a cultured oral cancer cell line using short interfering RNA (siRNA). Oral squamous carcinoma cell lines, HSC3 and SAS, were transfected with siRNA targeting HBEGF. Expression of HB‐EGF was analyzed by real‐time PCR. The invasiveness of the transfected cells was determined using a matrigel invasion assay, and MMP‐9 production was measured by RTPCR and gelatin zymography. The expression of HB‐EGF was reduced in HSC3‐siRNA and SAS‐siRNA cells. The matrigel invasion assay demonstrated that the invasiveness of HSC3‐siRNA and SAS‐siRNA cells was reduced. Gelatin zymography demonstrated that in HSC3‐siRNA and SAS‐siRNA cells, MMP9 production was decreased. These findings suggest that HB‐EGF expression is related to the invasion activity of oral cancer, particularly via regulation of MMP9.