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The Relationship between Subcellular Localization of Actinin‐4 and Cell Motility in Oral Squamous Cell Carcinoma
Author(s) -
Iida Yutaka,
Chiba Hiroshige
Publication year - 2004
Publication title -
oral science international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.256
H-Index - 13
eISSN - 1881-4204
pISSN - 1348-8643
DOI - 10.1016/s1348-8643(04)80004-1
Subject(s) - motility , cell , subcellular localization , actin , cell culture , microbiology and biotechnology , biology , cell type , cytoskeleton , actinin , chemistry , cytoplasm , biochemistry , genetics
To elucidate the mechanism of invasion of oral squamous cell carcinoma, we newly established two different cell lines with a high‐motility phenotype (designated HI type) and low‐motility phenotype (LI type) from CA‐9‐22, a human oral squamous cell carcinoma cell line, through cell invasion assay (Boyden chamber assay). When we examined the subcellular localization and protein expression of actinin‐4 using these cell lines, although the growth curves were not significantly different between the HI type and LI type, more invasion was seen in the HI‐type than in the LI‐type on Boyden chamber assay (p < 0.0001). Morphologically, a larger number of sharply extended cell processes and spindle formation were observed in the HI‐type than in the LI‐type, and actinin‐4 was mainly distributed in these processes. Western analysis showed that the expression level of actinin‐4 was almost equivalent between the HI and LI types. These findings suggest that subcellular localization of actinin‐4 might be involved in cell motility and cancer invasion by regulating the actin cytoskeleton at the cell processes in oral squamous cell carcinoma.