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Integrin Expression and Migration of Adenoid Cystic Carcinoma Cells in Response to Basement Membrane Components
Author(s) -
Cui-Ying Li,
Abu-Ali Samah,
Sugiura Tsuyoshi,
Shiratsuchi Toru,
Sasaki Masanori,
Shirasuna Kanemitsu
Publication year - 2004
Publication title -
oral science international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.256
H-Index - 13
eISSN - 1881-4204
pISSN - 1348-8643
DOI - 10.1016/s1348-8643(04)80003-x
Subject(s) - antibody dependent cell mediated cytotoxicity , integrin , basement membrane , cell culture , motility , adenoid cystic carcinoma , biology , collagen receptor , cell , type iv collagen , microbiology and biotechnology , cancer research , immunology , chemistry , laminin , carcinoma , extracellular matrix , antibody , biochemistry , genetics , monoclonal antibody
Adenoid cystic carcinoma (AdCC) is more aggressive than oral squamous cell carcinoma (SCC) and has a proclivity to invade nerve and endothelial sheaths containing basement membrane (BM). In the present study, we found that AdCC cell lines showed higher motility responses to BM proteins, especially type IV collagen, compared to oral SCC cell lines. Flow cytometric analysis demonstrated that AdCC cell lines showed generally similar pattern of integrin subunits to oral SCC lines. Although AdCC cell lines expressed slightly higher levels of α 2 β 1 and α 3 β 1 integrins than SCC cell lines, these differences were not as impressive as those seen in haptotaxis assays to ECM proteins, and in particular to type IV collagen. Antibody blocking experiments clearly showed that the AdCC motility response to type IV collagen is mainly mediated through α 2 β 1 integrin. In conclusion, such high motility response of AdCC cell lines could not be explained by only the surface levels of integrins, and study on integrin functionality will be more important to elucidate high invasiveness of AdCC cells.