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The expression of matrix metalloproteinase‐13 and osteocalcin in mouse osteoblasts is related to osteoblastic differentiation and is modulated by 1,25‐dihydroxyvitamin D3 and thyroid hormones
Author(s) -
FratzlZelman Nadja,
Glantschnig Helmut,
Rumpler Monika,
Nader Alexander,
Ellinger Adolf,
Varga Franz
Publication year - 2003
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/s1065-6995(03)00037-4
Subject(s) - osteocalcin , osteoblast , endocrinology , medicine , matrix metalloproteinase , chemistry , hormone , runx2 , thyronine , bone remodeling , bone cell , parathyroid hormone , alkaline phosphatase , type i collagen , cellular differentiation , matrix (chemical analysis) , microbiology and biotechnology , biology , calcium , thyroid hormones , biochemistry , enzyme , in vitro , gene , chromatography
Matrix metalloproteinase‐13 (MMP‐13), is a key protein of bone matrix degradation, and is highly expressed by osteoblasts. We used the osteoblast‐like MC3T3‐E1 cell line and compared the stimulatory effects of the bone resorptive agents 1,25‐dihydroxyvitamin D3 (1,25‐(OH) 2 D 3 ) 3,3′,5‐triido‐ l ‐thyronine (T3) on the expression of MMP‐13 mRNA. We showed that the stimulatory effects were time and dose dependent, and were also transduced to the protein level, with 1,25‐(OH) 2 D 3 being more potent. MMP‐13 expression in different mouse cells and its localization within developing bone from the onset of osteogenesis were also investigated. 1,25‐(OH) 2 D 3 ‐ and T3‐regulated osteocalcin (Osc) expression in mouse osteoblasts was compared to hormonal effects on MMP‐13 expression and activity. Here we show divergent and common roles of 1,25‐(OH) 2 D 3 and T3 action on the expression of these marker proteins, depending on the stage of cell differentiation. In addition, we propose a role for MMP‐13 in the bone collar of developing long bones. The results could help to more precisely characterize hormonal regulation in the developmental sequence of osteoblasts.