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Unexpected durability of PKH 26 staining on rat adipocytes
Author(s) -
Rieck Bernd
Publication year - 2003
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/s1065-6995(03)00036-2
Subject(s) - staining , adipose tissue , transplantation , persistence (discontinuity) , connective tissue , cell , fluorescent staining , microbiology and biotechnology , chemistry , biology , pathology , medicine , endocrinology , biochemistry , geotechnical engineering , engineering
In a case of autologous mature fat cell transplantation to an individual rat, persistence of adipose staining with PKH26 was detected 14.5 months later. Fluorescent fat cells with spotty surface markings were easily visible. Such a long period of persistence and stability of staining exceeds the expectation from previous reports, and the given half‐life of the substance. This finding encourages the use of the dye for long‐term follow‐up of connective tissue cells, especially adipocytes and preadipocytes, following transplantation.