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Modulation of glutamate neurotoxicity on mesencephalic dopaminergic neurons in primary cultures by the presence of striatal target cells
Author(s) -
Mateu Guylaine,
Privat Alain,
Thibault Jean,
Vig Jacques
Publication year - 2000
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/s0736-5748(99)00110-0
Subject(s) - glutamate receptor , dopaminergic , substantia nigra , striatum , nmda receptor , neurotoxicity , biology , cerebellum , population , tyrosine hydroxylase , dopamine , neurotoxin , chemistry , neuroscience , receptor , toxicity , endocrinology , biochemistry , medicine , environmental health , organic chemistry
Glutamate toxicity was compared in substantia nigra (SN)/striatum (STR) and SN/cerebellum (CRB) co‐cultures on both the entire neuronal population (neuron specific enolase (NSE) immunopositive cells) and dopaminergic neurons (tyrosine hydroxylase (TH) immunopositive cells). In SN/CRB co‐cultures NSE‐ and TH‐positive cells were more sensitive to glutamate‐induced toxicity than in SN/STR co‐cultures. Moreover, in SN/STR co‐cultures as compared to SN/CRB and SN cultures, glutamate toxicity was prevented to a larger extent by TCP, a non‐competitive NMDA antagonist. These results suggest that target cells induce a differential expression of the different glutamate receptor subtypes in mesencephalic dopaminergic cells. Alternatively, the presence of target cells may induce the selective development of a subpopulation of dopaminergic neurons expressing predominantly NMDA receptors.